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Home / ELISA Kit

Human Parathyroid Pormone 1 Receptor (PTH1R) ELISA Kit

SKU : BHE13703281

Parathyroid hormone receptor 1 functions as a receptor for parathyroid hormone (PTH) and for parathyroid hormone-like hormone (PTHLH).This "classical" PTH receptor is expressed in high levels in bone and kidney and regulates calcium ion homeostasis through activation of adenylate cyclase and phospholipase C. In bone, it is expressed on the surface of osteoblasts. When the receptor is activated, these cells in turn stimulate osteoclasts to ultimately increase the resorption rate.It is a member of the secretin family of G protein-coupled receptors. The activity of this receptor is mediated by Gs G proteins which activate adenylyl cyclase. Besides, they also activate phosphatidylinositol-calcium second messenger system.Expressed in most tissues. Most abundant in kidney, bone and liver.

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Mfr.No.	KTE61065
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Parathyroid Pormone 1 Receptor (PTH1R) ELISA Kit employs a two-site sandwich ELISA to quantitate PTH1R.
Alternative Names	PTH1R; MGC138426; MGC138452; PFE; PTHR; PTHR1; OTTHUMP00000209970; OTTHUMP00000209971; PTH/PTHr receptor; PTH/PTHrP type I receptor; parathyroid hormone receptor 1; parathyroid hormone/parathyroid hormone-relat
Detection Method	Colorimetric
Kit Components	Human Parathyroid Pormone 1 Receptor microplate Human Parathyroid Pormone 1 Receptor standard Human Parathyroid Pormone 1 Receptor detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Parathyroid Pormone 1 Receptor (PTH1R) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTH1R. No significant cross-reactivity or interference between Human PTH1R and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Parathyroid hormone receptor 1 functions as a receptor for parathyroid hormone (PTH) and for parathyroid hormone-like hormone (PTHLH).This "classical" PTH receptor is expressed in high levels in bone and kidney and regulates calcium ion homeostasis through activation of adenylate cyclase and phospholipase C. In bone, it is expressed on the surface of osteoblasts. When the receptor is activated, these cells in turn stimulate osteoclasts to ultimately increase the resorption rate.It is a member of the secretin family of G protein-coupled receptors. The activity of this receptor is mediated by Gs G proteins which activate adenylyl cyclase. Besides, they also activate phosphatidylinositol-calcium second messenger system.Expressed in most tissues. Most abundant in kidney, bone and liver.
Notes	This Human Parathyroid Pormone 1 Receptor (PTH1R) ELISA Kit employs a two-site sandwich ELISA to quantitate PTH1R in samples. An antibody specific for PTH1R has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTH1R present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTH1R is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTH1R bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5745

Applications	ELISA
Applications Notes	This Human Parathyroid Pormone 1 Receptor (PTH1R) ELISA Kit employs a two-site sandwich ELISA to quantitate PTH1R in samples. An antibody specific for PTH1R has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTH1R present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTH1R is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTH1R bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703281	KTE61065	48 T	$359		Add to Cart
BHE13703281	KTE61065	96 T	$619		Add to Cart
BHE13703281	KTE61065	96 Tx5	$2809		Add to Cart
BHE13703281	KTE61065	96 Tx50	$26209		Add to Cart

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