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Home / ELISA Kit

Human Parathyroid hormone 2 receptor (PTH2R) ELISA Kit

SKU : BHE13703280

Parathyroid hormone receptor 2 is a protein encoded by this gene is a member of the G-protein coupled receptor family 2. This protein is a receptor for parathyroid hormone (PTH). This receptor is more selective in ligand recognition and has a more specific tissue distribution compared to parathyroid hormone receptor 1 (PTHR1). There is 30 to 70% amino acid sequence identity between receptors within the secretin receptor family, but these receptors have essentially no sequence identity with most known G protein-coupled receptors of the rhodopsin family or with the metabotropic glutamate receptor family. PTHR2 is most similar (51% overall amino acid identity) to the PTH/PTHRP receptor. The PTHR2 gene was most abundantly expressed in brain, pancreas, testis, and placenta.

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Mfr.No.	KTE61064
Description	This Human Parathyroid hormone 2 receptor (PTH2R) ELISA Kit employs a two-site sandwich ELISA to quantitate PTH2R.
Alternative Names	PTH2R; PTHR2; PTH2 receptor; parathyroid hormone receptor 2
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Parathyroid hormone 2 receptor microplate Human Parathyroid hormone 2 receptor standard Human Parathyroid hormone 2 receptor detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Parathyroid hormone 2 receptor (PTH2R) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTH2R. No significant cross-reactivity or interference between Human PTH2R and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Parathyroid hormone receptor 2 is a protein encoded by this gene is a member of the G-protein coupled receptor family 2. This protein is a receptor for parathyroid hormone (PTH). This receptor is more selective in ligand recognition and has a more specific tissue distribution compared to parathyroid hormone receptor 1 (PTHR1). There is 30 to 70% amino acid sequence identity between receptors within the secretin receptor family, but these receptors have essentially no sequence identity with most known G protein-coupled receptors of the rhodopsin family or with the metabotropic glutamate receptor family. PTHR2 is most similar (51% overall amino acid identity) to the PTH/PTHRP receptor. The PTHR2 gene was most abundantly expressed in brain, pancreas, testis, and placenta.
Notes	This Human Parathyroid hormone 2 receptor (PTH2R) ELISA Kit employs a two-site sandwich ELISA to quantitate PTH2R in samples. An antibody specific for PTH2R has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTH2R present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTH2R is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTH2R bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	5746
Antigen	PTH2R
Tag	PTH2R
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Parathyroid hormone 2 receptor (PTH2R) ELISA Kit employs a two-site sandwich ELISA to quantitate PTH2R in samples. An antibody specific for PTH2R has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTH2R present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTH2R is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTH2R bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703280	KTE61064	48 T	$339		Add to Cart
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