Human Pappalysin-2(PAPPA2) ELISA kit

Mfr.No.

CSB-EL017436HU

Sensitivity

0.5 ng/mL

Detection Range

2 ng/mL-60 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

pappalysin 2, RP4-774I24.1, PAPP-A2, PAPPE, PLAC3, placenta-specific 3, pregnancy-associated plasma preproprotein-A2, pregnancy-associated plasma protein-E

Recovery

The recovery of human IL-23A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	94-101
EDTA plasma (n=4)	107	100-114

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-23A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	83-97
1:2	Average %	97
	Range %	91-104
1:4	Average %	84
	Range %	81-88
1:8	Average %	89
	Range %	81-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	1.762	1.845	1.804	1.628
2000	1.001	1.022	1.012	0.836
1000	0.669	0.667	0.668	0.492
500	0.407	0.416	0.412	0.236
250	0.297	0.277	0.287	0.111
125	0.253	0.246	0.250	0.074
62.5	0.220	0.216	0.218	0.042
0	0.172	0.179	0.176	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q9BXP8

Applications	ELISA
Research Area	PAPPA2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503686	CSB-EL017436HU	96T	$695	Add to Cart
BHE10503686	CSB-EL017436HU	5x96T	$3336	Add to Cart
BHE10503686	CSB-EL017436HU	10x96T	$6602.50	Add to Cart

Human Pappalysin-2(PAPPA2) ELISA kit

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