Human P4HA1(Prolyl 4-hydroxylase subunit alpha-1) ELISA Kit

Mfr.No.

EH1228

Alternative Names

4-PH alpha-1 ELISA Kit, Prolyl 4-hydroxylase subunit alpha-1 ELISA Kit, P4HA1 ELISA Kit, P4HA ELISA Kit, Procollagen-proline ELISA Kit, 2-oxoglutaRate-4-dioxygenase subunit alpha-1 ELISA Kit

Reactivity

Human

Detection Method

Double Antibody

Detection Range

0.625-40ng/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of P4HA1 and the recovery rates were calculated by comparing the measured value to the expected amount of P4HA1 in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	89-105	98
EDTA plasma(n=5)	88-101	95
heparin plasma(n=5)	86-105	95

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of P4HA1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	85-104%	96-99%	89-102%
EDTA plasma(n=5)	82-99%	84-101%	84-98%
heparin plasma(n=5)	80-94%	82-98%	81-92%

Sensitivity

< 0.375ng/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

4-PH alpha-1(Prolyl 4-hydroxylase subunit alpha-1)/P4HA1/P4HA/Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1

Notes

For Research Use Only

UniProt ID	P13674
Antigen	P4HA1

Applications	ELISA
Applications Notes	P4HA1 ELISA Kit allows for the in vitro quantitative determination of P4HA1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10806854	EH1228	96T	$400		Add to Cart

Human P4HA1(Prolyl 4-hydroxylase subunit alpha-1) ELISA Kit

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