Human P2X purinoceptor 7(P2RX7) ELISA kit

Mfr.No.

CSB-EL017325HU

Alternative Names

purinergic receptor P2X, ligand-gated ion channel, 7, MGC20089, P2X7, ATP receptor, P2X purinoceptor 7, P2X7 receptor, P2Z receptor, purinergic receptor P2X7

Reactivity

Human

Detection Range

25 pg/mL-1600 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human IL22RA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	98-105
EDTA plasma (n=4)	98	93-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IL22RA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	94
	Range %	87-98
1:2	Average %	107
	Range %	103-112
1:4	Average %	91
	Range %	85-97
1:8	Average %	92
	Range %	88-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	1.927	2.057	1.992	1.889
1000	1.342	1.460	1.401	1.298
500	0.922	0.965	0.944	0.841
250	0.651	0.663	0.657	0.554
125	0.432	0.412	0.422	0.319
62.5	0.254	0.269	0.262	0.159
31.25	0.208	0.201	0.205	0.102
0	0.104	0.102	0.103	?

Species

Homo sapiens

Sensitivity

6.25 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q99572
Antigen	purinergic receptor P2X, ligand-gated ion channel, 7

Applications

ELISA

Research Area

P2RX7

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503667	CSB-EL017325HU	96T	$695	Add to Cart
BHE10503667	CSB-EL017325HU	5x96T	$3336	Add to Cart
BHE10503667	CSB-EL017325HU	10x96T	$6602.50	Add to Cart

Human P2X purinoceptor 7(P2RX7) ELISA kit

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