Matrices listed below were spiked with certain level of p-tau217 and the recovery rates were calculated by comparing the measured value to the expected amount of p-tau217 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
94-100
97
EDTA plasma(n=5)
87-104
97
heparin plasma(n=5)
85-101
90
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of p-tau217 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-102%
85-99%
88-102%
EDTA plasma(n=5)
89-99%
86-99%
86-98%
heparin plasma(n=5)
88-100%
88-99%
91-99%
Sensitivity
< 4.688pg/ml
Notes
For Research Use Only
UniProt ID
P10636
Applications
ELISA
Applications Notes
p-tau217 ELISA Kit allows for the in vitro quantitative determination of p-tau217 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human p-tau217(Phospho Tau 217) ELISA Kit