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Home / ELISA Kit

Human P-Selectin (SELP) ELISA Kit

SKU : BHE13703307

P-selectin is the largest of the known selectins at 140kDa. It contains nine consensus repeats (CR) and extends approximately 40 nm from the endothelial surface. P-selectin is expressed in a-granules of activated platelets and granules of endothelial cells. Within minutes of stimulation of the endothelial cells by inflammatory mediators such as histamine, thrombin, or phorbol esters, P-selectin is surface-expressed. Expression of P-selectin also occurs from the surgical trauma endured during preparation of the tissues for intravital microscopy. The expression is short-lived, reaching its peak after only ten minutes. Additional synthesis of P-selectin is brought about within two hours by cytokines such as interleukin-1 (IL-1) or tumor necrosis factor a (TNF-a).

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Mfr.No.	KTE61091
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human P-Selectin (SELP) ELISA Kit employs a two-site sandwich ELISA to quantitate SELP.
Alternative Names	SELP; RP1-86F14.2; CD62; CD62P; FLJ45155; GMP140; GRMP; LECAM3; PADGEM; PSEL; OTTHUMP00000032609; granulocyte membrane protein; leukocyte-endothelial cell adhesion molecule 3; platelet alpha-granule membrane
Detection Method	Colorimetric
Kit Components	Human P-Selectin microplate Human P-Selectin standard Human P-Selectin detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human P-Selectin (SELP) ELISA Kit has high sensitivity and excellent specificity for detection of Human SELP. No significant cross-reactivity or interference between Human SELP and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	P-selectin is the largest of the known selectins at 140kDa. It contains nine consensus repeats (CR) and extends approximately 40 nm from the endothelial surface. P-selectin is expressed in a-granules of activated platelets and granules of endothelial cells. Within minutes of stimulation of the endothelial cells by inflammatory mediators such as histamine, thrombin, or phorbol esters, P-selectin is surface-expressed. Expression of P-selectin also occurs from the surgical trauma endured during preparation of the tissues for intravital microscopy. The expression is short-lived, reaching its peak after only ten minutes. Additional synthesis of P-selectin is brought about within two hours by cytokines such as interleukin-1 (IL-1) or tumor necrosis factor a (TNF-a).
Notes	This Human P-Selectin (SELP) ELISA Kit employs a two-site sandwich ELISA to quantitate SELP in samples. An antibody specific for SELP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySELP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SELP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SELP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	281486

Applications	ELISA
Applications Notes	This Human P-Selectin (SELP) ELISA Kit employs a two-site sandwich ELISA to quantitate SELP in samples. An antibody specific for SELP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySELP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SELP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SELP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703307	KTE61091	48 T	$399		Add to Cart
BHE13703307	KTE61091	96 T	$739		Add to Cart
BHE13703307	KTE61091	96 Tx5	$3339		Add to Cart
BHE13703307	KTE61091	96 Tx50	$29639		Add to Cart

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