Human Oxysterol Binding Protein Like Protein 5

Mfr.No.

E3364Hu

Sensitivity

0.049ng/ml

Detection Range

0.1-50ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Oxysterol Binding Protein Like Protein 5 (also known as OSBPL5) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

OBPH1;ORP5;ORP-5;OSBPL5;OSBP-related protein 5;Oxysterol-binding protein homolog 1;Oxysterol-binding protein-related protein 5

Molecular Weight

98616

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3364Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	17.5	0.8	4.56
Intra-Assay	2	18	7.91	0.27	3.38
Intra-Assay	3	18	6.83	0.3	4.38

Background

Lipid transporter involved in lipid countertransport between the endoplasmic reticulum and the plasma membrane: specifically exchanges phosphatidylserine with phosphatidylinositol 4-phosphate (PI4P), delivering phosphatidylserine to the plasma membrane in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum. Binds phosphatidylserine and PI4P in a mutually exclusive manner (PubMed:23934110, PubMed:26206935). May cooperate with NPC1 to mediate the exit of cholesterol from endosomes/lysosomes (PubMed:21220512). Binds 25-hydroxycholesterol and cholesterol (PubMed:17428193).

Protocol

These standard curves of E3364Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
28ng/ml	2.171	2.155	2.114
	2.139
14ng/ml	1.552	1.529	1.488
	1.506
7ng/ml	0.646	0.681	0.641
	0.717
3.5ng/ml	0.317	0.311	0.27
	0.305
1.75ng/ml	0.152	0.156	0.115
	0.159
0ng/ml	0.047	0.041	0
	0.035

Gene ID	114879
UniProt ID	Q9H0X9

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104893	E3364Hu	48T	$320		Add to Cart
BHE12104893	E3364Hu	96T	$458		Add to Cart

Human Oxysterol Binding Protein Like Protein 5 - OSBPL5 ELISA Kit

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