Human Oxysterol Binding Protein Like Protein 2

Mfr.No.

E3365Hu

Description

This sandwich kit is for the accurate quantitative detection of Human Oxysterol Binding Protein Like Protein 2 (also known as OSBPL2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

DFNA67;DNFA67;ORP2;ORP-2;OSBPL2;OSBP-related protein 2;Oxysterol-binding protein-related protein 2

Reactivity

Human

Detection Range

0.05-20ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

55201

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.02ng/ml

Background

Intracellular transport protein that binds sterols and phospholipids and mediates lipid transport between intracellular compartments. Increases plasma membrane cholesterol levels and decreases phosphatidylinositol-4,5-bisphosphate levels in the cell membrane (PubMed:30581148). Binds phosphoinositides, such as phosphatidylinositol-4,5-bisphosphate (PubMed:30581148). Exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate (PubMed:11279184). Binds cholesterol, dehydroergosterol, 22(R)-hydroxycholesterol and 25-hydroxycholesterol (in vitro) (PubMed:17428193, PubMed:19224871, PubMed:30581148).

Gene ID	9885
UniProt ID	Q9H1P3
Antigen	OSBPL2

Research Area

Others

Protocol

These standard curves of E3365Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.316	2.333	2.299
	2.35
6ng/ml	1.395	1.336	1.301
	1.276
3ng/ml	0.727	0.776	0.742
	0.825
1.5ng/ml	0.418	0.463	0.429
	0.508
0.75ng/ml	0.195	0.226	0.192
	0.257
0ng/ml	0.036	0.034	0
	0.033

Precision

We measured random samples of E3365Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	5.03	0.3	5.9
Intra-Assay	2	18	8.49	0.3	3.48
Intra-Assay	3	18	1.65	0.1	5.92

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104894	E3365Hu	48T	$320		Add to Cart
BHE12104894	E3365Hu	96T	$458		Add to Cart

Human Oxysterol Binding Protein Like Protein 2 - OSBPL2 ELISA Kit

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