Human Oxysterol Binding Protein Like Protein 10

Mfr.No.

E3366Hu

Description

This sandwich kit is for the accurate quantitative detection of human Oxysterol Binding Protein Like Protein 10 (also known as OSBPL10) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

ORP10;ORP-10;OSBP9;OSBPL10;OSBP-related protein 10;Oxysterol-binding protein-related protein 10

Reactivity

Human

Detection Range

0.05-15ng/ml

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

83970

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.027ng/ml

Background

Probable lipid transporter involved in lipid countertransport between the endoplasmic reticulum and the plasma membrane. Its ability to bind phosphatidylserine, suggests that it specifically exchanges phosphatidylserine with phosphatidylinositol 4-phosphate (PI4P), delivering phosphatidylserine to the plasma membrane in exchange for PI4P (PubMed:23934110) (Probable). Plays a role in negative regulation of lipid biosynthesis (PubMed:19554302). Negatively regulates APOB secretion from hepatocytes (PubMed:19554302, PubMed:22906437). Binds cholesterol and acidic phospholipids (PubMed:22906437). Also binds 25-hydroxycholesterol (PubMed:17428193). Binds phosphatidylserine (PubMed:23934110).

Gene ID	114884
UniProt ID	Q9BXB5
Antigen	OSBPL10

Research Area

Others

Protocol

These standard curves of E3366Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	2.204	2.228	2.183
	2.252
4ng/ml	1.575	1.554	1.509
	1.532
2ng/ml	0.609	0.612	0.567
	0.616
1ng/ml	0.344	0.361	0.316
	0.377
0.5ng/ml	0.202	0.169	0.124
	0.136
0ng/ml	0.033	0.045	0
	0.057

Precision

We measured random samples of E3366Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.4	0.09	2.66
Intra-Assay	2	18	3.21	0.11	3.48
Intra-Assay	3	18	1.98	0.08	3.96

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104895	E3366Hu	48T	$320		Add to Cart
BHE12104895	E3366Hu	96T	$458		Add to Cart

Human Oxysterol Binding Protein Like Protein 10 - OSBPL10 ELISA Kit

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