Human oxidized low density lipoprotein,OxLDL ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E07931h

Sensitivity

0.78 mU/mL

Detection Range

1.56 mU/mL-100 mU/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

oxidized lowdensity lipoprotein,OxLDL

Recovery

The recovery of mouse CRF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-96
EDTA plasma (n=4)	94	87-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CRF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-100
1:2	Average %	97
	Range %	90-105
1:4	Average %	96
	Range %	95-102
1:8	Average %	94
	Range %	90-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	OxLDL

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Green tea epigallocatechin-3-gallate attenuates Porphyromonas gingivalis-induced atherosclerosis Cai Y et al,Pathog Dis,2013 Feb;67(1):76-83. Association between Serum Ferritin and Circulating Oxidized Low-density Lipoprotein Levels in Patients with Coronary Artery Disease Ikeda Y et al,Endocr J,2006 Oct;53(5):665-70. Levels of oxidized LDL, estrogens and progesterone in placenta tissues and serum paraoxonase activity in preeclampsia Accedilikgoumlz S et al,Mediators Inflamm,2013:862982. Endothelial overexpression of LOX-1 increases plaque formation and promotes atherosclerosis in vivo Akhmedov A et al,Eur Heart J,Jan 12. Homoarginine supplementation improves blood glucose in diet-induced obese mice Stockebrand M. et al,Amino Acids. ,2015 Sep;47(9):1921-9. Curcumin pretreatment protects against PM2.5 Shi J.et al,Mol Med Rep,Jul 6 Localization of lipopolysaccharide from Escherichia Coli into human atherosclerotic plaque Carnevale R.et al,Sci Rep,Feb 26

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