Human oxidized low density lipoprotein antibody,OLAb ELISA Kit

Mfr.No.

CSB-E08797h

Sensitivity

11.75 uU/mL

Detection Range

47 uU/mL-3000 uU/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

oxidized lowdensity lipoprotein antibody,OLAb

Recovery

The recovery of human GR-? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	87	83-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GR-? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	103
	Range %	99-108
1:2	Average %	98
	Range %	94-102
1:4	Average %	91
	Range %	88-95
1:8	Average %	89
	Range %	85-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	ox-LDL Ab

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Cameroonian professional soccer players and risk of atherosclerosis Nansseu JR.et al,BMC Res Notes,Jun 2

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