Human oxidized high density lipoprotein (Ox-HDL)ELISA Kit

Mfr.No.

CSB-E16552h

Alternative Names

oxidized high density lipoprotein (Ox-HDL)

Reactivity

Human

Detection Range

31.25 pg/mL-2000 pg/mL

Sample Type

Serum, Plasma, Urine, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of pig IL-22 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	91-103
EDTA plasma (n=4)	101	95-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-22 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	99
	Range %	94-104
1:2	Average %	96
	Range %	89-100
1:4	Average %	95
	Range %	89-98
1:8	Average %	103
	Range %	95-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

7.81 pg/mL

Limit Of Detection

450 nm

Antigen	oxidized high density lipoprotein (Ox-HDL)

Applications

ELISA

Research Area

Ox-HDL

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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