Matrices listed below were spiked with certain level of OVCA2 and the recovery rates were calculated by comparing the measured value to the expected amount of OVCA2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-105
96
EDTA plasma(n=5)
87-96
92
heparin plasma(n=5)
96-104
100
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OVCA2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-105%
86-102%
86-104%
EDTA plasma(n=5)
82-94%
83-99%
88-98%
heparin plasma(n=5)
82-95%
81-97%
82-97%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
Q8WZ82
Applications
ELISA
Applications Notes
OVCA2 ELISA Kit allows for the in vitro quantitative determination of OVCA2 concentrations in serum, plasma, tissue homogenates and other biological fluids.