Human ovalbumin specific IgE,OVA sIgE ELISA Kit

Human ovalbumin specific IgE,OVA sIgE ELISA Kit

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Mfr.No.

CSB-E09740h

Alternative Names

ovalbumin specific IgE,OVA sIgE

Reactivity

Human

Detection Range

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Sample Type

Serum, Plasma

Assay Type

Indirect

Assay Duration

1-5h

Recovery

The recovery of human CREB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	93-102
EDTA plasma (n=4)	86	80-92

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CREB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	92
	Range %	87-96
1:10	Average %	89
	Range %	85-94
1:20	Average %	95
	Range %	89-98
1:40	Average %	102
	Range %	98-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.157	2.254	2.206	2.104
10	1.715	1.812	1.764	1.662
5	1.267	1.284	1.276	1.174
2.5	0.857	0.863	0.860	0.758
1.25	0.523	0.532	0.528	0.426
0.625	0.343	0.351	0.347	0.245
0.312	0.206	0.213	0.210	0.108
0	0.103	0.101	0.102

Species

Homo sapiens

Sensitivity

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Limit Of Detection

450 nm

Antigen	ovalbumin specific IgE,OVA sIgE

Applications

ELISA

Research Area

OVA sIgE

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	A rat model of exercise-induced asthma: a nonspecific response to a specific immunogen /,american journal of physiology-regulatory integrative and comparative physiology,2011 Apr;300(4):R917-24.

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SKU	Supplier No.	Size	Your price
BHE10503655	CSB-E09740h	96T	$790	Add to Cart
BHE10503655	CSB-E09740h	5x96T	$3792	Add to Cart
BHE10503655	CSB-E09740h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.