Human Outer Dense Fiber Protein 4

Mfr.No.

E4665Hu

Description

This sandwich kit is for the accurate quantitative detection of human Outer Dense Fiber Protein 4 (also known as ODF4) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

hOPPO1;ODF 4;ODF4;Outer dense fiber of sperm tails protein 4;Outer dense fiber protein 4;Testis-specific protein oppo 1

Reactivity

Human

Detection Range

10-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

29233

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.18ng/L

Background

Component of the outer dense fibers (ODF) of spermatozoa which could be involved in sperm tail structure, sperm movement and general organization of cellular cytoskeleton.

Gene ID	146852
UniProt ID	Q2M2E3
Antigen	ODF4

Research Area

Epigenetics and Nuclear Signaling

Protocol

These standard curves of E4665Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.311	2.264	2.207
	2.218
600ng/L	1.716	1.67	1.614
	1.625
300ng/L	1.184	1.084	1.027
	0.984
150ng/L	0.771	0.725	0.669
	0.679
75ng/L	0.415	0.454	0.398
	0.494
0ng/L	0.064	0.057	0
	0.05

Precision

We measured random samples of E4665Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	765.4	42.55	5.6
Intra-Assay	2	18	283.6	21.46	7.6
Intra-Assay	3	18	64.3	3.91	6.1

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105961	E4665Hu	48T	$320		Add to Cart
BHE12105961	E4665Hu	96T	$458		Add to Cart

Human Outer Dense Fiber Protein 4 - ODF4 ELISA Kit

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