Human Osteoprotegerin,OPG ELISA KIT

Mfr.No.

CSB-E04692h

Sensitivity

1.95 pg/mL

Detection Range

7.8 pg/mL-500 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

tumor necrosis factor receptor superfamily, member 11b, MGC29565, OCIF, OPG, TR1, osteoclastogenesis inhibitory factor, osteoprotegerin

Recovery

The recovery of human S100A10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	105	103-109
EDTA plasma (n=4)	96	93-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human S100A10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	101
	Range %	97-104
1:2	Average %	108
	Range %	104-112
1:4	Average %	95
	Range %	91-98
1:8	Average %	89
	Range %	85-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	O00300

Applications	ELISA
Research Area	TNFRSF11B

Storage	Store at 2-8°C. Please refer to protocol.

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