Human Osteocalcin/Bone gla protein,OT/BGP ELISA kit

Human Osteocalcin/Bone gla protein,OT/BGP ELISA kit

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Mfr.No.

CSB-E05128h

Sensitivity

7.8 pg/ml

Detection Range

31.25 pg/ml-2000 pg/ml

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

bone gamma-carboxyglutamate (gla) protein, RP11-54H19.5, BGP, OC, PMF1, bone Gla protein, bone gamma-carboxyglutamate (gla) protein (osteocalcin), gamma-carboxyglutamic acid-containing protein, osteocalcin

Recovery

The recovery of human GAD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-95
EDTA plasma (n=4)	99	95-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GAD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	93
	Range %	90-98
1:2	Average %	86
	Range %	82-93
1:4	Average %	95
	Range %	90-100
1:8	Average %	92
	Range %	87-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.512	2.458	2.485	2.357
10	1.678	1.788	1.733	1.605
5	1.105	1.190	1.148	1.020
2.5	0.685	0.710	0.698	0.570
1.25	0.474	0.465	0.470	0.342
0.625	0.340	0.321	0.331	0.203
0.312	0.240	0.221	0.231	0.103
0	0.125	0.131	0.128	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P02818

Applications	ELISA
Research Area	BGLAP

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503646	CSB-E05128h	96T	$790	Add to Cart
BHE10503646	CSB-E05128h	5x96T	$3792	Add to Cart
BHE10503646	CSB-E05128h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.