Human orphanin FQ/nociceptin,OFQ/N ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E09741h

Sensitivity

1.17 pg/mL

Detection Range

4.69 pg/mL-300 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

rphanin FQ/nociceptin, PPNOC, nocistatin, propronociceptin

Recovery

The recovery of human COL3A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-98
EDTA plasma (n=4)	109	102-115

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human COL3A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-96
1:2	Average %	100
	Range %	95-106
1:4	Average %	101
	Range %	95-106
1:8	Average %	96
	Range %	90-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q13519

Applications	ELISA
Research Area	PNOC

Storage	Store at 2-8°C. Please refer to protocol.

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