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Home / ELISA Kit

Human Orexigenic neuropeptide QRFP (QRFP) ELISA Kit

SKU : BHE13703218

P518 functions as a high-affinity ligand of GPR103. Both GPR103 and P518 precursor mRNA exhibited highest expression in brain.The 43-amino acid QRFP peptide, a longer form of the P518 peptide is necessary to exhibit full agonistic activity with GPR103. Intravenous administration QRFP caused release of aldosterone, suggesting that QRFP and GPR103 regulate adrenal function.The deduced 126-amino acid protein contains a putative N-terminal 22-amino acid signal peptide and no transmembrane domain, suggesting that the protein or cleavage products can be secreted. The 26-amino acid P518 peptide sequence is located at the C terminus of the precursor protein. In peripheral tissues, P518 precursor mRNA was detected in prostate, testis, colon, thyroid, parathyroid, coronary artery, and bladder.

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Mfr.No.	KTE61002
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Orexigenic neuropeptide QRFP (QRFP) ELISA Kit employs a two-site sandwich ELISA to quantitate QRFP.
Alternative Names	QRFP; RP11-618A20.5; 26RFa; MGC119794; P518; P518 precursor protein; RF(Arg-Phe)amide family 26 amino acid peptide; RF(Arg-Phe)amide family 26 amino acid peptide (P518)
Detection Method	Colorimetric
Kit Components	Human Orexigenic neuropeptide QRFP microplate Human Orexigenic neuropeptide QRFP standard Human Orexigenic neuropeptide QRFP detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Orexigenic neuropeptide QRFP (QRFP) ELISA Kit has high sensitivity and excellent specificity for detection of Human QRFP. No significant cross-reactivity or interference between Human QRFP and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	P518 functions as a high-affinity ligand of GPR103. Both GPR103 and P518 precursor mRNA exhibited highest expression in brain.The 43-amino acid QRFP peptide, a longer form of the P518 peptide is necessary to exhibit full agonistic activity with GPR103. Intravenous administration QRFP caused release of aldosterone, suggesting that QRFP and GPR103 regulate adrenal function.The deduced 126-amino acid protein contains a putative N-terminal 22-amino acid signal peptide and no transmembrane domain, suggesting that the protein or cleavage products can be secreted. The 26-amino acid P518 peptide sequence is located at the C terminus of the precursor protein. In peripheral tissues, P518 precursor mRNA was detected in prostate, testis, colon, thyroid, parathyroid, coronary artery, and bladder.
Notes	This Human Orexigenic neuropeptide QRFP (QRFP) ELISA Kit employs a two-site sandwich ELISA to quantitate QRFP in samples. An antibody specific for QRFP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyQRFP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for QRFP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of QRFP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	347148

Applications	ELISA
Applications Notes	This Human Orexigenic neuropeptide QRFP (QRFP) ELISA Kit employs a two-site sandwich ELISA to quantitate QRFP in samples. An antibody specific for QRFP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyQRFP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for QRFP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of QRFP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703218	KTE61002	48 T	$339		Add to Cart
BHE13703218	KTE61002	96 T	$589		Add to Cart
BHE13703218	KTE61002	96 Tx5	$2629		Add to Cart
BHE13703218	KTE61002	96 Tx50	$24569		Add to Cart

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