OPN N-half ELISA Kit, cleaved form Osteopontin ELISA Kit
Reactivity
Human
Detection Method
Double Antibody
Detection Range
0.156-10ng/ml
Sample Type
Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of OPN N-half and the recovery rates were calculated by comparing the measured value to the expected amount of OPN N-half in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-104
94
EDTA plasma(n=5)
85-99
93
heparin plasma(n=5)
88-105
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OPN N-half and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
94-101%
86-104%
90-105%
EDTA plasma(n=5)
86-100%
86-93%
85-97%
heparin plasma(n=5)
86-100%
85-98%
85-100%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
OPN N-half ELISA Kit allows for the in vitro quantitative determination of OPN N-half concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human OPN N-half (cleaved form Osteopontin) ELISA Kit