Matrices listed below were spiked with certain level of Opi and the recovery rates were calculated by comparing the measured value to the expected amount of Opi in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-105
97
EDTA plasma(n=5)
86-97
91
heparin plasma(n=5)
94-100
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Opi and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Opi ELISA Kit allows for the in vitro quantitative determination of Opi concentrations in serum, plasma, tissue homogenates and other biological fluids.