Human OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine®

Mfr.No.

EK0480

Sensitivity

<5 pg/ml

Detection Range

93.7 pg/ml - 6,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	93.7	187.5	375	750	1500	3000	6000
O.D.	0.002	0.081	0.163	0.282	0.550	0.841	1.506	2.019

Description

Human OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine® (96 Tests). Quantitate Human TNFRSF11B in cell culture supeatants, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Tumor necrosis factor receptor superfamily member 11B;Osteoclastogenesis inhibitory factor;Osteoprotegerin;TNFRSF11B;OCIF, OPG;

Molecular Weight

46026 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: E22-L401

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human OPG

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma, Saliva, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	132	650	2696	130	692	2678
Standard deviation	10.42	37.7	113.23	11.57	51.2	136.57
CV (%)	7.9%	5.8%	4.2%	8.9%	7.4%	5.1%

Protein Function

Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis. .

Background

Osteoprotegerin (OPG) is identical to osteoclastogenesis inhibitory factor (OCIF), a soluble member of the tumor-necrosis factor receptor family that inhibits osteoclastogenesis. OPG is considered to play an important role in the regulation of bone resorption by modifying osteoclast differentiation. Osteoprotegerin is an independent risk factor for the progression of atherosclerosis and onset of cardiovascular disease. It can act as a soluble factor in the regulation of bone mass and imply a utility for OPG in the treatment of osteoporosis associated with increased osteoclast activity. OPG system may play a critical role in the development of osteolytic bone disease in multiple myeloma and that targeting this system may have therapeutic potential. OPG also plays a role in the vascular system. The standard product used in this kit is recombinant OPG, a 55KDa glycoprotein.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	132	142	137	133	136	3.93	2.8%
Sample 2	650	625	651	585	627	26.78	4.2%
Sample 3	2696	3024	2657	2825	2800	143.23	5.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	4982
Gene Name	TNFRSF11B
UniProt ID	O00300
Protein Name	Tumor necrosis factor receptor superfamily member 11B
Tissue Specificity	Highly expressed in adult lung, heart, kidney, liver, spleen, thymus, prostate, ovary, small intestine, thyroid, lymph node, trachea, adrenal gland, testis, and bone marrow. Detected at very low levels in brain, placenta and skeletal muscle. Highly expressed in fetal kidney, liver and lung.
Sequence	Contains 2 death domains.

Applications	ELISA
Research Area	Atherosclerosis,Cardiovascular,Cytokines,Cytoskeleton/ECM,Extracellular Matrix,Immunology,Innate Immunity,Metabolism,Obesity,Signal Transduction,Structures,TNF Superfamily,Vascular Inflammation

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000470	EK0480	96 wells/kit	$499		Add to Cart

Human OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine®

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