Human Oncomodulin ELISA Kit PicoKine®

Mfr.No.

EK1545

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.4	125	250	500	1,000	2,000
O.D.	0.011	0.058	0.112	0.212	0.413	0.769	1.422	2.138

Description

Human Oncomodulin ELISA Kit PicoKine® (96 Tests). Quantitate Human OCM in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Oncomodulin-1;OM;Parvalbumin beta;OCM;OCM1, OCMN;

Molecular Weight

12184 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S2-S109

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Oncomodulin

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	102	272	897	105	292	975
Standard deviation	5.5	20.67	63.68	7.14	25.4	85.8
CV (%)	5.4%	7.6%	7.1%	6.8%	8.7%	8.8%

Protein Function

Has some calmodulin-like activity with respect to enzyme activation and growth regulation. Binds two calcium ions.

Background

Oncomodulin, also known as OCM, is an oncodevelopmental protein found in early embryonic cells in the placenta and also in tumors. The human gene for oncomodulin is located on chromosome 7. It is a parvalbumin- family calcium-binding protein expressed and secreted by macrophages that typically traffic to tissue as an inflammatory response or after injury. Oncomodulin promotes axon regeneration in retinal ganglion cells and maintains functioning in mouse cochlear hair cells.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	102	101	94	100	99	3.11	3.1%
Sample 2	272	264	287	282	276	8.89	3.2%
Sample 3	897	877	847	824	861	27.91	3.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	654231
Gene Name	OCM
UniProt ID	P0CE72
Protein Name	Oncomodulin-1

Applications	ELISA
Research Area	Calcium Binding Proteins,Calcium Signaling,Signal Transduction,Signaling Pathway

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001070	EK1545	96 wells/kit	$750		Add to Cart

Human Oncomodulin ELISA Kit PicoKine®

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