Human ON(Osteonectin) ELISA Kit

Mfr.No.

EH3478

Alternative Names

ON ELISA Kit, BM-40 ELISA Kit, Basement-membrane protein 40 ELISA Kit, BM-40 ELISA Kit, ONcysteine-rich protein ELISA Kit, Secreted protein acidic and rich in cysteine ELISA Kit, secreted protein ELISA Kit, acidic ELISA Kit, cysteine-rich ELISA Kit, osteonectin ELISA Kit

Reactivity

Human

Detection Method

Double Antibody

Detection Range

0.156-10ng/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of ON and the recovery rates were calculated by comparing the measured value to the expected amount of ON in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	87-104	98
EDTA plasma(n=5)	86-104	97
heparin plasma(n=5)	86-102	94

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ON and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	85-102%	86-105%	88-103%
EDTA plasma(n=5)	89-91%	82-91%	82-99%
heparin plasma(n=5)	83-100%	85-98%	83-95%

Sensitivity

< 0.094ng/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

ON/BM-40/Basement-membrane protein 40/BM-40/ONcysteine-rich protein/Secreted protein acidic and rich in cysteine/secreted protein, acidic, cysteine-rich(osteonectin)

Notes

For Research Use Only

UniProt ID	P09486
Antigen	ON

Applications	ELISA
Applications Notes	ON ELISA Kit allows for the in vitro quantitative determination of ON concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10803976	EH3478	96T	$400		Add to Cart

Human ON(Osteonectin) ELISA Kit

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