Human Oligodendrocyte Transcription Factor 1

Mfr.No.

E0695Hu

Description

This sandwich kit is for the accurate quantitative detection of human Oligodendrocyte Transcription Factor 1 (also known as Olig1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

BHLHB6;BHLHE21;Class B basic helix-loop-helix protein 6;Class E basic helix-loop-helix protein 21;Olig1;Oligo1;Oligodendrocyte transcription factor 1

Reactivity

Human

Detection Range

0.5-150ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

27905

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.025ng/ml

Background

Promotes formation and maturation of oligodendrocytes, especially within the brain. Cooperates with OLIG2 to establish the pMN domain of the embryonic neural tube (By similarity).

Gene ID	116448
UniProt ID	Q8TAK6
Antigen	Olig1

Research Area

Neuroscience

Protocol

These standard curves of E0695Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
80ng/ml	2.33	2.167	2.12
	2.005
40ng/ml	1.406	1.393	1.346
	1.381
20ng/ml	0.933	0.877	0.829
	0.82
10ng/ml	0.418	0.448	0.401
	0.478
5ng/ml	0.249	0.246	0.199
	0.243
0ng/ml	0.043	0.048	0
	0.052

Precision

We measured random samples of E0695Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	7.38	0.39	5.26
Intra-Assay	2	18	59.77	2.35	3.94
Intra-Assay	3	18	20.98	1.14	5.43

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102377	E0695Hu	48T	$320		Add to Cart
BHE12102377	E0695Hu	96T	$458		Add to Cart

Human Oligodendrocyte Transcription Factor 1 - OLIG1 ELISA Kit

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