Human Olfactomedin-2/Noelin-2 ELISA Kit PicoKine®

Mfr.No.

EK2272

Sensitivity

<25 pg/ml

Detection Range

46.9 pg/ml - 3,000 pg/ml

Reactivity

Human

Sample Volume

Concentration(pg/ml)	0	46.9	93.8	187.5	375	750	1,500	3,000
O.D.	0.023	0.039	0.116	0.262	0.439	0.876	1.341	1.867

Description

Human Olfactomedin-2/Noelin-2 ELISA Kit PicoKine® (96 Tests). Quantitate Human Olfactomedin-2/Noelin-2 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 25 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q21-P454

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human OLFM2

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	49	139	970	59	123	838
Standard deviation	1.86	6.27	46.66	2.94	7.49	31.26
CV(%)	3.8%	4.5%	4.8%	5%	6.1%	3.7%

Background

Olfactomedin 2, also known as noelin 2, is a protein that in humans is encoded by the OLFM2 gene. OLFM2 is an olfactomedin domain-containing protein that is involved in early eye development and function. It also plays a role in TGFB-mediated differentiation of smooth muscle cells (SMCs) from mesenchymal stem cells.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	49	62	49	64	56	1.25	2.2%
Sample 2	139	107	110	187	136	4.91	3.6%
Sample 3	970	911	1371	1205	1114	71.87	6.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	93145
Gene Name	OLFM2
UniProt ID	O95897

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001821	EK2272	96 wells/kit	$750		Add to Cart

Human Olfactomedin-2/Noelin-2 ELISA Kit PicoKine®

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