Matrices listed below were spiked with certain level of OCT and the recovery rates were calculated by comparing the measured value to the expected amount of OCT in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-101
92
EDTA plasma(n=5)
89-105
94
heparin plasma(n=5)
87-103
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OCT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
OCT ELISA Kit allows for the in vitro quantitative determination of OCT concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human OCT (Semaphorin receptor OCT) ELISA Kit