Human Occludin - OCLN ELISA Kit

Mfr.No.

E3658Hu

Sensitivity

0.27ng/ml

Detection Range

0.5-150ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Occludin (also known as OCLN) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Occludin;OCLN

Molecular Weight

59144

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3658Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	24.9	1.27	5.1
Intra-Assay	2	18	38.7	1.02	2.6
Intra-Assay	3	18	9.7	0.58	6

Background

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. (Microbial infection) Acts as a co-receptor for hepatitis C virus (HCV) in hepatocytes.

Protocol

These standard curves of E3658Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
80ng/ml	2.429	2.353	2.334
	2.276
40ng/ml	1.272	1.314	1.295
	1.357
20ng/ml	0.79	0.753	0.734
	0.715
10ng/ml	0.386	0.406	0.387
	0.427
5ng/ml	0.123	0.146	0.126
	0.168
0ng/ml	0.014	0.019	0
	0.024

Gene ID	100506658
UniProt ID	Q16625

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105132	E3658Hu	48T	$320		Add to Cart
BHE12105132	E3658Hu	96T	$458		Add to Cart

Human Occludin - OCLN ELISA Kit

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