Human Obestatin ELISA Kit

Human Obestatin ELISA Kit

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Mfr.No.

CSB-E12818h

Detection Range

4.69 pg/mL-300 pg/mL

Sensitivity

1.17 pg/mL

Reactivity

Human

Sample Type

Serum, Cell culture supernatants, Urine, Tissue homogenates, Plasma

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Obestatin

Recovery

The recovery of rat CPB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	93	88-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat CPB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	95-110
1:2	Average %	84
	Range %	80-88
1:4	Average %	98
	Range %	92-103
1:8	Average %	105
	Range %	100-112

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
800	2.596	2.714	2.655	2.536
400	2.212	2.142	2.177	2.058
200	1.455	1.524	1.490	1.371
100	0.789	0.765	0.777	0.658
50	0.421	0.448	0.435	0.316
25	0.243	0.253	0.248	0.129
12.5	0.165	0.175	0.170	0.051
0	0.120	0.118	0.119

Species

Homo sapiens

Limit Of Detection

450 nm

Antigen	Obestatin

Applications

ELISA

Research Area

Obestatin

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Is association between thyroid hormones and gut peptides, ghrelin and obestatin, able to suggest new regulatory relation between the HPT axis and gut? Emami A et al,Regul Pept,2014 Feb 4;189C:17-21. Acute effect of smoking and smoking abstinence on energy intake and appetite-related hormones blood concentrations Yannakoulia M.et al,Physiol Behav,Nov 8

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.