Human Nucleotide-binding oligomerization domain-containing protein 2(NOD2) ELISA kit

Human Nucleotide-binding oligomerization domain-containing protein 2(NOD2) ELISA kit

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Mfr.No.

CSB-EL015915HU

Alternative Names

nucleotide-binding oligomerization domain containing 2, ACUG, BLAU, CARD15, CD, CLR16.3, IBD1, NLRC2, NOD2B, PSORAS1, NLR family, CARD domain containing 2, NOD-like receptor C2, caspase recruitment domain family, member 15, caspase recruitment domain protei

Reactivity

Human

Detection Range

25 pg/mL-1600 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rabbit IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-98
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	100
	Range %	91-105
1:4	Average %	97
	Range %	92-105
1:8	Average %	94
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
40	2.804	2.786	2.795	2.706
20	2.280	2.176	2.228	2.139
10	1.544	1.443	1.494	1.405
5	0.899	0.876	0.888	0.799
2.5	0.443	0.423	0.433	0.344
1.25	0.230	0.226	0.228	0.139
0.625	0.154	0.149	0.152	0.063
0	0.090	0.088	0.089	?

Species

Homo sapiens

Sensitivity

6.25 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q9HC29
Antigen	nucleotide-binding oligomerization domain containing 2

Applications

ELISA

Research Area

NOD2

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503621	CSB-EL015915HU	96T	$695	Add to Cart
BHE10503621	CSB-EL015915HU	5x96T	$3336	Add to Cart
BHE10503621	CSB-EL015915HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.