Human Nucleotide-binding oligomerization domain-containing protein 1(NOD1) ELISA kit

Human Nucleotide-binding oligomerization domain-containing protein 1(NOD1) ELISA kit

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Mfr.No.

CSB-EL015914HU

Alternative Names

nucleotide-binding oligomerization domain containing 1, CARD4, CLR7.1, NLRC1, NLR family, CARD domain containing 1, caspase recruitment domain family, member 4, caspase recruitment domain-containing protein 4, nucleotide-binding oligomerization domain, leuc

Reactivity

Human

Detection Range

15.6 pg/mL-1000 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of pig IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-105
EDTA plasma (n=4)	92	86-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-91
1:2	Average %	90
	Range %	85-95
1:4	Average %	89
	Range %	84-93
1:8	Average %	96
	Range %	92-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.719	2.652	2.686	2.492
200	1.962	1.861	1.912	1.718
100	1.213	1.204	1.209	1.015
50	0.748	0.689	0.719	0.525
25	0.551	0.527	0.539	0.345
12.5	0.355	0.374	0.365	0.171
6.25	0.295	0.272	0.284	0.090
0	0.192	0.196	0.194	?

Species

Homo sapiens

Sensitivity

3.9 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q9Y239
Antigen	nucleotide-binding oligomerization domain containing 1

Applications

ELISA

Research Area

NOD1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503620	CSB-EL015914HU	96T	$695	Add to Cart
BHE10503620	CSB-EL015914HU	5x96T	$3336	Add to Cart
BHE10503620	CSB-EL015914HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.