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Home / ELISA Kit

Human Nuclease-sensitive element-binding protein 1 (YBX1) ELISA Kit

SKU : BHE13702229

Y box binding protein 1is a human protein. Current research is examining its involvement in cancer, and particularly in the metastasis of cancerous cells or prevention thereof.
The deduced 322-amino acid protein contains 4 putative DNA-binding domains, 3 of which are rich in basic amino acids. Two of these basic regions, basic-1 and basic-2, form the double-strand DNA-binding domain of the protein. NSEP1 also has a pro/ser/thr-rich domain and an asp/glu/gln-rich domain, both of which are reminiscent of activation domains. It also contains an octapeptide single-strand DNA-binding motif that shares weak homology with the ribonucleoprotein consensus sequence of RNA-binding proteins.

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Mfr.No.	KTE60009
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Nuclease-sensitive element-binding protein 1 (YBX1) ELISA Kit employs a two-site sandwich ELISA to quantitate YBX1.
Alternative Names	YBX1; BP-8; CSDA2; CSDB; DBPB; MDR-NF1; MGC104858; MGC110976; MGC117250; NSEP-1; NSEP1; YB-1; YB1; DNA-binding protein B; major histocompatibility complex; class; Y box-binding protein I; nuclease sensit
Detection Method	Colorimetric
Kit Components	Human Nuclease-sensitive element-binding protein 1 microplate Human Nuclease-sensitive element-binding protein 1 standard Human Nuclease-sensitive element-binding protein 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Nuclease-sensitive element-binding protein 1 (YBX1) ELISA Kit has high sensitivity and excellent specificity for detection of Human YBX1. No significant cross-reactivity or interference between Human YBX1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Y box binding protein 1is a human protein. Current research is examining its involvement in cancer, and particularly in the metastasis of cancerous cells or prevention thereof. The deduced 322-amino acid protein contains 4 putative DNA-binding domains, 3 of which are rich in basic amino acids. Two of these basic regions, basic-1 and basic-2, form the double-strand DNA-binding domain of the protein. NSEP1 also has a pro/ser/thr-rich domain and an asp/glu/gln-rich domain, both of which are reminiscent of activation domains. It also contains an octapeptide single-strand DNA-binding motif that shares weak homology with the ribonucleoprotein consensus sequence of RNA-binding proteins.
Notes	This Human Nuclease-sensitive element-binding protein 1 (YBX1) ELISA Kit employs a two-site sandwich ELISA to quantitate YBX1 in samples. An antibody specific for YBX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyYBX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YBX1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YBX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	4904

Applications	ELISA
Applications Notes	This Human Nuclease-sensitive element-binding protein 1 (YBX1) ELISA Kit employs a two-site sandwich ELISA to quantitate YBX1 in samples. An antibody specific for YBX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyYBX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YBX1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YBX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702229	KTE60009	48 T	$339		Add to Cart
BHE13702229	KTE60009	96 T	$589		Add to Cart
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