Human Nuclease-sensitive element-binding protein 1(YBX1) ELISA kit

Mfr.No.

CSB-EL026247HU

Alternative Names

Y box binding protein 1, BP-8, CSDA2, CSDB, DBPB, MDR-NF1, MGC104858, MGC110976, MGC117250, NSEP-1, NSEP1, YB-1, YB1, DNA-binding protein B, major histocompatibility complex, class II, Y box-binding protein I, nuclease sensit

Reactivity

Human

Detection Range

31.25 pg/mL-2000 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of horse HIF1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-102
EDTA plasma (n=4)	103	92-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of horse HIF1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-94
1:2	Average %	102
	Range %	96-107
1:4	Average %	92
	Range %	87-98
1:8	Average %	94
	Range %	89-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














pg/ml	OD1	OD2	Average	Corrected
2000	1.899	1.957	1.928	1.819
1000	1.417	1.496	1.457	1.348
500	0.926	0.978	0.952	0.843
250	0.583	0.572	0.578	0.469
125	0.345	0.353	0.349	0.240
62.5	0.246	0.252	0.249	0.140
31.25	0.171	0.178	0.175	0.066
0	0.108	0.109	0.109	?

Species

Homo sapiens

Sensitivity

7.8 pg/mL

Limit Of Detection

450 nm

UniProt ID	P67809
Antigen	Y box binding protein 1

Applications

ELISA

Research Area

YBX1

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503613	CSB-EL026247HU	96T	$695	Add to Cart
BHE10503613	CSB-EL026247HU	5x96T	$3336	Add to Cart
BHE10503613	CSB-EL026247HU	10x96T	$6602.50	Add to Cart

Human Nuclease-sensitive element-binding protein 1(YBX1) ELISA kit

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