Human Nuclear receptor subfamily 4 group A member 2(NR4A2) ELISA kit

Human Nuclear receptor subfamily 4 group A member 2(NR4A2) ELISA kit

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Mfr.No.

CSB-EL016063HU

Alternative Names

nuclear receptor subfamily 4, group A, member 2, HZF-3, NOT, NURR1, RNR1, TINUR, NGFI-B/nur77 beta-type transcription factor homolog, OTTHUMP00000204707, T-cell nuclear receptor NOT, intermediate-early receptor protein, nur related protein-1, human ho

Reactivity

Human

Detection Range

31.25 pg/mL-2000 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of sheep IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-103
EDTA plasma (n=4)	95	89-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	93
	Range %	90-98
1:2	Average %	96
	Range %	92-99
1:4	Average %	94
	Range %	89-101
1:8	Average %	92
	Range %	88-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
0	2.541	2.439	2.490
2	1.382	1.352	1.367
6	0.756	0.785	0.771
18.4	0.405	0.412	0.409
40	0.219	0.224	0.222
80	0.126	0.123	0.125

Species

Homo sapiens

Sensitivity

7.8 pg/mL

Limit Of Detection

450 nm

UniProt ID	P43354
Antigen	nuclear receptor subfamily 4, group A, member 2

Applications

ELISA

Research Area

NR4A2

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10503612	CSB-EL016063HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.