Human Nuclear receptor subfamily 4 group A member 2(NR4A2) ELISA kit

Human Nuclear receptor subfamily 4 group A member 2(NR4A2) ELISA kit

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Mfr.No.

CSB-EL016063HU

Sensitivity

7.8 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

nuclear receptor subfamily 4, group A, member 2, HZF-3, NOT, NURR1, RNR1, TINUR, NGFI-B/nur77 beta-type transcription factor homolog, OTTHUMP00000204707, T-cell nuclear receptor NOT, intermediate-early receptor protein, nur related protein-1, human ho

Recovery

The recovery of sheep IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-103
EDTA plasma (n=4)	95	89-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	93
	Range %	90-98
1:2	Average %	96
	Range %	92-99
1:4	Average %	94
	Range %	89-101
1:8	Average %	92
	Range %	88-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
0	2.541	2.439	2.490
2	1.382	1.352	1.367
6	0.756	0.785	0.771
18.4	0.405	0.412	0.409
40	0.219	0.224	0.222
80	0.126	0.123	0.125

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P43354

Applications	ELISA
Research Area	NR4A2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10503612	CSB-EL016063HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.