Human Nuclear receptor ROR-gamma(RORC) ELISA kit

Human Nuclear receptor ROR-gamma(RORC) ELISA kit

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Mfr.No.

CSB-EL020071HU

Alternative Names

RAR-related orphan receptor C, MGC129539, NR1F3, RORG, RZR-GAMMA, RZRG, TOR, RAR-related orphan receptor C, isoform a, RAR-related orphan receptor gamma, nuclear receptor ROR-gamma, retinoic acid-binding receptor gamma, retinoid-rela

Reactivity

Human

Detection Range

12.5 pg/mL-800 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat IL-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	94	91-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	91
	Range %	88-93
1:2	Average %	104
	Range %	99-107
1:4	Average %	93
	Range %	88-97
1:8	Average %	99
	Range %	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	1.854	1.766	1.810	1.727
250	1.117	1.106	1.112	1.029
125	0.728	0.743	0.736	0.653
62.5	0.517	0.534	0.526	0.443
31.2	0.303	0.312	0.308	0.225
15.6	0.210	0.202	0.206	0.123
7.8	0.171	0.164	0.168	0.085
0	0.084	0.082	0.083	?

Species

Homo sapiens

Sensitivity

3.12 pg/mL

Limit Of Detection

450 nm

UniProt ID	P51449
Antigen	RAR-related orphan receptor C

Applications

ELISA

Research Area

RORC

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503611	CSB-EL020071HU	96T	$695	Add to Cart
BHE10503611	CSB-EL020071HU	5x96T	$3336	Add to Cart
BHE10503611	CSB-EL020071HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.