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Home / ELISA Kit

Human Nuclear receptor coactivator 3 (NCOA3) ELISA Kit

SKU : BHE13703558

Nuclear receptor coactivator 3 encoded by NCOA3 is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. Nuclear receptor coactivator 3 has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. Nuclear receptor coactivator 3 is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Several transcript variants encoding different isoforms have been found for NCOA3. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein.

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Mfr.No.	KTE61343
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Nuclear receptor coactivator 3 (NCOA3) ELISA Kit employs a two-site sandwich ELISA to quantitate NCOA3.
Alternative Names	NCOA3; RP5-1049G16.3; ACTR; AIB-1; AIB1; CAGH16; CTG26; KAT13B; MGC141848; RAC3; SRC-3; SRC3; TNRC14; TNRC16; TRAM-1; bHLHe42; pCIP; CBP-interacting protein; OTTHUMP00000031716; amplified in breast cancer-1;
Detection Method	Colorimetric
Kit Components	Human Nuclear receptor coactivator 3 microplate Human Nuclear receptor coactivator 3 standard Human Nuclear receptor coactivator 3 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Nuclear receptor coactivator 3 (NCOA3) ELISA Kit has high sensitivity and excellent specificity for detection of Human NCOA3. No significant cross-reactivity or interference between Human NCOA3 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Nuclear receptor coactivator 3 encoded by NCOA3 is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. Nuclear receptor coactivator 3 has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. Nuclear receptor coactivator 3 is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Several transcript variants encoding different isoforms have been found for NCOA3. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein.
Notes	This Human Nuclear receptor coactivator 3 (NCOA3) ELISA Kit employs a two-site sandwich ELISA to quantitate NCOA3 in samples. An antibody specific for NCOA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNCOA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NCOA3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NCOA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	8202

Applications	ELISA
Applications Notes	This Human Nuclear receptor coactivator 3 (NCOA3) ELISA Kit employs a two-site sandwich ELISA to quantitate NCOA3 in samples. An antibody specific for NCOA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNCOA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NCOA3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NCOA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703558	KTE61343	48 T	$339		Add to Cart
BHE13703558	KTE61343	96 T	$589		Add to Cart
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BHE13703558	KTE61343	96 Tx50	$24569		Add to Cart

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