Human Nuclear Receptor Coactivator 3

Mfr.No.

E5795Hu

Alternative Names

ACTR;AIB1;AIB-1;Amplified in breast cancer 1 protein;bHLHe42;CAGH16;CBP-interacting protein;Class E basic helix-loop-helix protein 42;KAT13B;NCOA3;NCoA-3;Nuclear receptor coactivator 3;pCIP;RAC3;RAC-3;Receptor-associated coactivator 3;SRC3;SRC-3;Steroid receptor coactivator protein 3;Thyroid hormone receptor activator molecule 1;TNRC14;TNRC16;TRAM-1

Reactivity

Human

Detection Range

10-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

155293

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.48ng/L

Background

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.

Gene ID	8202
UniProt ID	Q9Y6Q9
Antigen	NCOA3

Research Area

Epigenetics and Nuclear Signaling; Signal Transduction; Cancer; Metabolism

Protocol

These standard curves of E5795Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	1.724	1.853	1.813
	1.982
600ng/L	1.006	0.988	0.948
	0.97
300ng/L	0.624	0.633	0.593
	0.642
150ng/L	0.24	0.241	0.201
	0.241
75ng/L	0.067	0.091	0.051
	0.115
0ng/L	0.025	0.04	0
	0.055

Precision

We measured random samples of E5795Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	306.34	17.45	5.7
Intra-Assay	2	18	831.9	28.43	3.42
Intra-Assay	3	18	617	27.97	4.53

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107157	E5795Hu	48T	$320		Add to Cart
BHE12107157	E5795Hu	96T	$458		Add to Cart

Human Nuclear Receptor Coactivator 3 - NCOA3 ELISA Kit

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