Human Nuclear Factor of Activated T-cells

Mfr.No.

E6695Hu

Sensitivity

0.023ng/ml

Detection Range

0.05-10ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

NFAT pre-existing subunit;NFAT1;NFATC2;NF-ATc2;NFATP;NF-ATp;Nuclear factor of activated T-cells, cytoplasmic 2;T-cell transcription factor NFAT1

Molecular Weight

100146

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E6695Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.57	0.12	3.47
Intra-Assay	2	18	0.84	0.07	7.73
Intra-Assay	3	18	2.55	0.12	4.73

Background

Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway.

Protocol

These standard curves of E6695Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
6.4ng/ml	2.672	2.645	2.595
	2.617
3.2ng/ml	1.49	1.446	1.397
	1.402
1.6ng/ml	0.929	0.898	0.849
	0.868
0.8ng/ml	0.524	0.492	0.442
	0.459
0.4ng/ml	0.273	0.241	0.192
	0.209
0ng/ml	0.038	0.05	0
	0.062

Gene ID	4773
UniProt ID	Q13469

Research Area	Immunology; Signal Transduction; Epigenetics and Nuclear Signaling; Cardiovascular; Cancer; Metabolism

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115305	E6695Hu	48T	$320		Add to Cart
BHE12115305	E6695Hu	96T	$458		Add to Cart

Human Nuclear Factor of Activated T-cells - NFATC2 ELISA Kit

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