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Home / ELISA Kit

Human Nuclear factor-KB p65 (NF-KB p65) ELISA Kit

SKU : BHE13703027

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of RELA. Four transcript variants encoding different isoforms have been found for RELA.

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Mfr.No.	KTE60811
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Nuclear factor-KB p65 (NF-KB p65) ELISA Kit employs a two-site sandwich ELISA to quantitate RELA.
Alternative Names	RELA; MGC131774; NFKB3; p65; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene
Detection Method	Colorimetric
Kit Components	Human Nuclear factor-KB p65 microplate Human Nuclear factor-KB p65 standard Human Nuclear factor-KB p65 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Nuclear factor-KB p65 (NF-KB p65) ELISA Kit has high sensitivity and excellent specificity for detection of Human RELA. No significant cross-reactivity or interference between Human RELA and analogues was observed.
Calibration Range	Please inquire
Sample Type	Serum, Cell culture supeatants, Plasma, Other biological fluids
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of RELA. Four transcript variants encoding different isoforms have been found for RELA.
Notes	This Human Nuclear factor-KB p65 (NF-KB p65) ELISA Kit employs a two-site sandwich ELISA to quantitate RELA in samples. An antibody specific for RELA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRELA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RELA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RELA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5970

Applications	ELISA
Applications Notes	This Human Nuclear factor-KB p65 (NF-KB p65) ELISA Kit employs a two-site sandwich ELISA to quantitate RELA in samples. An antibody specific for RELA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRELA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RELA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RELA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703027	KTE60811	48 T	$359		Add to Cart
BHE13703027	KTE60811	96 T	$619		Add to Cart
BHE13703027	KTE60811	96 Tx5	$2809		Add to Cart
BHE13703027	KTE60811	96 Tx50	$26209		Add to Cart

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