Human Nuclear factor-kappa B,NF-kappaB ELISA Kit

Human Nuclear factor-kappa B,NF-kappaB ELISA Kit

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Mfr.No.

CSB-E12107h

Alternative Names

nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, DKFZp686C01211, EBP-1, KBF1, MGC54151, NF-kappa-B, NF-kappaB, NFKB-p105, NFKB-p50, p105, p50, DNA binding factor KBF1, NF-kappabeta, nuclear factor NF-kappa-B p50 subunit, nuclear factor kappa-B DNA bi

Reactivity

Human

Detection Range

0.312 ng/mL-20 ng/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat copeptin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	98-108
EDTA plasma (n=4)	88	82-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat copeptin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	96
	Range %	89-102
1:2	Average %	94
	Range %	91-99
1:4	Average %	99
	Range %	96-106
1:8	Average %	97
	Range %	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.916	2.815	2.866	2.742
2000	2.121	2.232	2.177	2.053
1000	1.390	1.401	1.396	1.272
500	0.800	0.810	0.805	0.681
250	0.458	0.447	0.453	0.329
125	0.263	0.252	0.258	0.134
62.5	0.173	0.171	0.172	0.048
0	0.124	0.123	0.124	?

Species

Homo sapiens

Sensitivity

0.078 ng/mL

Limit Of Detection

450 nm

UniProt ID	P19838
Antigen	nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

Applications

ELISA

Research Area

NFKB1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503602	CSB-E12107h	96T	$790	Add to Cart
BHE10503602	CSB-E12107h	5x96T	$3792	Add to Cart
BHE10503602	CSB-E12107h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.