Matrices listed below were spiked with certain level of NRTN and the recovery rates were calculated by comparing the measured value to the expected amount of NRTN in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-103
95
EDTA plasma(n=5)
88-102
95
heparin plasma(n=5)
88-103
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NRTN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
NRTN ELISA Kit allows for the in vitro quantitative determination of NRTN concentrations in serum, plasma, tissue homogenates and other biological fluids.