Matrices listed below were spiked with certain level of NRGN and the recovery rates were calculated by comparing the measured value to the expected amount of NRGN in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
92
EDTA plasma(n=5)
91-100
94
heparin plasma(n=5)
88-105
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NRGN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
NRGN ELISA Kit allows for the in vitro quantitative determination of NRGN concentrations in serum, plasma, tissue homogenates and other biological fluids.