Matrices listed below were spiked with certain level of NR2B and the recovery rates were calculated by comparing the measured value to the expected amount of NR2B in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-101
95
EDTA plasma(n=5)
91-103
96
heparin plasma(n=5)
87-100
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NR2B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-102%
89-100%
86-102%
EDTA plasma(n=5)
82-97%
84-90%
90-101%
heparin plasma(n=5)
80-100%
83-98%
81-100%
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Notes
For Research Use Only
UniProt ID
P19793
Applications
ELISA
Applications Notes
NR2BELISA Kit allows for the in vitro quantitative determination of NR2B concentrations in serum, plasma, tissue homogenates and other biological fluids.
Storage
2-8 °C for 6 months
Reviews of Human NR2B (Retinoic acid receptor RXR-alpha) ELISA Kit