Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of NOSTRIN and the recovery rates were calculated by comparing the measured value to the expected amount of NOSTRIN in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-100
95
EDTA plasma(n=5)
86-105
96
heparin plasma(n=5)
87-105
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NOSTRIN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-98%
86-102%
85-97%
EDTA plasma(n=5)
85-98%
89-100%
82-101%
heparin plasma(n=5)
84-95%
81-89%
82-100%
Sensitivity
< 0.188ng/ml
Notes
For Research Use Only
UniProt ID
Q8IVI9
Antigen
NOSTRIN
Applications
ELISA
Applications Notes
NOSTRIN ELISA Kit allows for the in vitro quantitative determination of NOSTRIN concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human NOSTRIN (Nitric oxide synthase traffic inducer) ELISA Kit