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Home / ELISA Kit

Human Non-receptor tyrosine-protein kinase TNK1 (TNK1) ELISA Kit

SKU : BHE13702415

TNK1 is a nonreceptor tyrosine kinase. These kinases, like members of the SRC and JAK families, mediate intracellular signaling downstream of receptor activation.
TNK1 contains a putative N-terminal phosphotyrosine-binding domain, followed by the tyrosine kinase domain, a putative SH3 domain, and a proline-rich C terminus that contains SH3-binding motifs. The kinase domain has all 12 structural features conserved in tyrosine kinases, including an ATP-binding site. TNK1 shares highest homology with ACK, including 45% amino acid identity over a 413-amino acid span that includes the kinase domain and putative SH3 domain. specific expression of 4 transcripts between 1.0 and 9.5 kb in fetal brain, lung, liver, and kidney.

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Mfr.No.	KTE60196
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Non-receptor tyrosine-protein kinase TNK1 (TNK1) ELISA Kit employs a two-site sandwich ELISA to quantitate TNK1.
Alternative Names	TNK1; MGC46193;
Detection Method	Colorimetric
Kit Components	Human Non-receptor tyrosine-protein kinase TNK1 microplate Human Non-receptor tyrosine-protein kinase TNK1 standard Human Non-receptor tyrosine-protein kinase TNK1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Non-receptor tyrosine-protein kinase TNK1 (TNK1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNK1. No significant cross-reactivity or interference between Human TNK1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TNK1 is a nonreceptor tyrosine kinase. These kinases, like members of the SRC and JAK families, mediate intracellular signaling downstream of receptor activation. TNK1 contains a putative N-terminal phosphotyrosine-binding domain, followed by the tyrosine kinase domain, a putative SH3 domain, and a proline-rich C terminus that contains SH3-binding motifs. The kinase domain has all 12 structural features conserved in tyrosine kinases, including an ATP-binding site. TNK1 shares highest homology with ACK, including 45% amino acid identity over a 413-amino acid span that includes the kinase domain and putative SH3 domain. specific expression of 4 transcripts between 1.0 and 9.5 kb in fetal brain, lung, liver, and kidney.
Notes	This Human Non-receptor tyrosine-protein kinase TNK1 (TNK1) ELISA Kit employs a two-site sandwich ELISA to quantitate TNK1 in samples. An antibody specific for TNK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	8711

Applications	ELISA
Applications Notes	This Human Non-receptor tyrosine-protein kinase TNK1 (TNK1) ELISA Kit employs a two-site sandwich ELISA to quantitate TNK1 in samples. An antibody specific for TNK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702415	KTE60196	48 T	$339		Add to Cart
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