Matrices listed below were spiked with certain level of NODAL and the recovery rates were calculated by comparing the measured value to the expected amount of NODAL in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-100
92
EDTA plasma(n=5)
89-102
94
heparin plasma(n=5)
89-103
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NODAL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
NODAL ELISA Kit allows for the in vitro quantitative determination of NODAL concentrations in serum, plasma, tissue homogenates and other biological fluids.