Matrices listed below were spiked with certain level of NMU and the recovery rates were calculated by comparing the measured value to the expected amount of NMU in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-102
96
EDTA plasma(n=5)
86-105
94
heparin plasma(n=5)
94-104
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NMU and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
NMU ELISA Kit allows for the in vitro quantitative determination of NMU concentrations in serum, plasma, tissue homogenates and other biological fluids.