Matrices listed below were spiked with certain level of NMS and the recovery rates were calculated by comparing the measured value to the expected amount of NMS in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-102
95
EDTA plasma(n=5)
86-103
94
heparin plasma(n=5)
87-105
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NMS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
NMS ELISA Kit allows for the in vitro quantitative determination of NMS concentrations in serum, plasma, tissue homogenates and other biological fluids.