Matrices listed below were spiked with certain level of NMB and the recovery rates were calculated by comparing the measured value to the expected amount of NMB in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-104
96
EDTA plasma(n=5)
85-102
92
heparin plasma(n=5)
87-101
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NMB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-105%
85-103%
85-105%
EDTA plasma(n=5)
87-95%
82-97%
84-95%
heparin plasma(n=5)
83-88%
84-99%
87-99%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
P08949
Applications
ELISA
Applications Notes
NMB ELISA Kit allows for the in vitro quantitative determination of NMB concentrations in serum, plasma, tissue homogenates and other biological fluids.